Figure 3.

Delineation of the amino acids important for the interaction between hUMP1 and the proteasome. (A) Schematic description of the constructs used. Construct I represents the full-length hUMP1. Construct II depicts hUMP1 lacking the C-terminal 11 amino acids. Constructs III–V represent versions of hUMP1 lacking amino acids 46–49 (construct III), amino acids 67–90 (construct IV), or amino acids 68–72 (construct V). Constructs I–V were transcribed and translated in vitro and subjected to immunoprecipitation either by the monoclonal antibody against proteasome subunit α2 (B) or by the anti-ha antibody (C). Immunoprecipitated material was separated on SDS/15% PAGE. Constructs used are indicated at the bottom of each lane.