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. 1991 Sep;29(9):2086–2088. doi: 10.1128/jcm.29.9.2086-2088.1991

Comparison of the Clearview Chlamydia test, Chlamydiazyme, and cell culture for detection of Chlamydia trachomatis in women with a low prevalence of infection.

M Skulnick 1, G W Small 1, A E Simor 1, D E Low 1, H Khosid 1, S Fraser 1, R Chua 1
PMCID: PMC270270  PMID: 1774342

Abstract

Two antigen detection systems, Clearview Chlamydia (Unipath Ltd., Bedford, United Kingdom) and Chlamydiazyme (Abbott Laboratories, North Chicago, Ill.), were compared with culture for the diagnosis of chlamydia infection in women attending gynecological clinics. Chlamydia trachomatis was isolated from 43 (4.5%) of the 965 women tested. In comparison with tissue culture, the Clearview Chlamydia and Chlamydiazyme tests had sensitivities of 79.0 and 74.4%, respectively, and both had a specificity of 99.6%. The results show that the Clearview Chlamydia test is comparable to Chlamydiazyme for the detection of C. trachomatis from endocervical specimens in a population with a low prevalence of infection.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arumainayagam J. T., Matthews R. S., Uthayakumar S., Clay J. C. Evaluation of a novel solid-phase immunoassay, Clearview Chlamydia, for the rapid detection of Chlamydia trachomatis. J Clin Microbiol. 1990 Dec;28(12):2813–2814. doi: 10.1128/jcm.28.12.2813-2814.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnes R. C., Katz B. P., Rolfs R. T., Batteiger B., Caine V., Jones R. B. Quantitative culture of endocervical Chlamydia trachomatis. J Clin Microbiol. 1990 Apr;28(4):774–780. doi: 10.1128/jcm.28.4.774-780.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bell T. A., Grayston J. T. Centers for Disease Control guidelines for prevention and control of Chlamydia trachomatis infections. Summary and Commentary. Ann Intern Med. 1986 Apr;104(4):524–526. doi: 10.7326/0003-4819-104-4-524. [DOI] [PubMed] [Google Scholar]
  4. Hammerschlag M. R., Gelling M., Roblin P. M., Worku M. Comparison of Kodak Surecell Chlamydia Test Kit with culture for the diagnosis of chlamydial conjunctivitis in infants. J Clin Microbiol. 1990 Jun;28(6):1441–1442. doi: 10.1128/jcm.28.6.1441-1442.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jones R. B., Van Der Pol B., Katz B. P. Effect of differences in specimen processing and passage technique on recovery of Chlamydia trachomatis. J Clin Microbiol. 1989 May;27(5):894–898. doi: 10.1128/jcm.27.5.894-898.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kellogg J. A., Seiple J. W., Murray C. L., Levisky J. S. Effect of endocervical specimen quality on detection of Chlamydia trachomatis and on the incidence of false-positive results with the Chlamydiazyme method. J Clin Microbiol. 1990 Jun;28(6):1108–1113. doi: 10.1128/jcm.28.6.1108-1113.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LeBar W., Herschman B., Jemal C., Pierzchala J. Comparison of DNA probe, monoclonal antibody enzyme immunoassay, and cell culture for the detection of Chlamydia trachomatis. J Clin Microbiol. 1989 May;27(5):826–828. doi: 10.1128/jcm.27.5.826-828.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lefebvre J., Laperrière H., Rousseau H., Massé R. Comparison of three techniques for detection of Chlamydia trachomatis in endocervical specimens from asymptomatic women. J Clin Microbiol. 1988 Apr;26(4):726–731. doi: 10.1128/jcm.26.4.726-731.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Magder L. S., Klontz K. C., Bush L. H., Barnes R. C. Effect of patient characteristics on performance of an enzyme immunoassay for detecting cervical Chlamydia trachomatis infection. J Clin Microbiol. 1990 Apr;28(4):781–784. doi: 10.1128/jcm.28.4.781-784.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mahony J., Castriciano S., Sellors J., Stewart I., Cunningham I., Landis S., Seidelman W., Grant L., Devlin C., Chernesky M. Diagnosis of Chlamydia trachomatis genital infections by cell culture and two enzyme immunoassays detecting different chlamydial antigens. J Clin Microbiol. 1989 Sep;27(9):1934–1938. doi: 10.1128/jcm.27.9.1934-1938.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moncada J., Schachter J., Bolan G., Engelman J., Howard L., Mushahwar I., Ridgway G., Mumtaz G., Stamm W., Clark A. Confirmatory assay increases specificity of the chlamydiazyme test for Chlamydia trachomatis infection of the cervix. J Clin Microbiol. 1990 Aug;28(8):1770–1773. doi: 10.1128/jcm.28.8.1770-1773.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moncada J., Schachter J., Shipp M., Bolan G., Wilber J. Cytobrush in collection of cervical specimens for detection of Chlamydia trachomatis. J Clin Microbiol. 1989 Aug;27(8):1863–1866. doi: 10.1128/jcm.27.8.1863-1866.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sellors J., Mahony J., Jang D., Pickard L., Castriciano S., Landis S., Stewart I., Seidelman W., Cunningham I., Chernesky M. Rapid, on-site diagnosis of chlamydial urethritis in men by detection of antigens in urethral swabs and urine. J Clin Microbiol. 1991 Feb;29(2):407–409. doi: 10.1128/jcm.29.2.407-409.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith J. W., Rogers R. E., Katz B. P., Brickler J. F., Lineback P. L., Van der Pol B., Jones R. B. Diagnosis of chlamydial infection in women attending antenatal and gynecologic clinics. J Clin Microbiol. 1987 May;25(5):868–872. doi: 10.1128/jcm.25.5.868-872.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Thompson S. E., Washington A. E. Epidemiology of sexually transmitted Chlamydia trachomatis infections. Epidemiol Rev. 1983;5:96–123. doi: 10.1093/oxfordjournals.epirev.a036266. [DOI] [PubMed] [Google Scholar]

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