Figure 3.

There are a number of ways to increase sensitivity of detection for low copy number (LCN) typing. The one displayed here is post-polymerase chain reaction (post-PCR) purification using microcon dialysis to remove ions that compete with DNA during electrokinetic injection. A known 250 pg DNA sample was analyzed under standard operating profile conditions. The same sample was diluted to 7.8 pg and 2 replicates were independently amplified using the AmpFlSTR® Profiler® PCR Amplification Kit (Applied Biosystems, Foster City, CA, USA). To increase sensitivity of detection, the 7.8 pg samples were subjected to post-PCR purification and a 4-fold increase of sample was used for analysis. The example shows the results for a subset of the full profile (D3S1358, vWA, and FGA loci). Typical stochastic effects are observed for the LCN samples.