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. Author manuscript; available in PMC: 2009 Jun 29.
Published in final edited form as: Mol Biosyst. 2009 Mar 27;5(5):529–541. doi: 10.1039/b819502a

Figure 4.

Figure 4

(a) RICs for oxidised/derivatised oxysterol [M]+ ions, m/z 550.4003, from cortex (upper trace) and spinal cord (lower trace). (b & c) MS3 (550→471→) spectra of chromatographic peaks eluting at (b) 4.42 min (24,25-dihydroxycholesterol), and (c) 4.83 min (24,27-dihydroxycholesterol) from the cortex sample. Data was acquired on the LTQ-Orbitrap. RICs (± 5 ppm) were generated using the Orbitrap detector, and MS3 spectra were recorded using the LTQ detector. Chromatographic conditions are described in the Experimental. Shown in supplementary Figure S4 are spectra of other oxysterols identified and where applicable spectra of authentic standards.