Fig. 2. Calcium ionophore treatment increases ferritin H mRNA.

Jurkat (A) or NIH3T3 cells (B) were treated for 24 h with 0, 1, 5 uM ionomycin, or an equimolar concentration of DMSO vehicle was added. Northern blots were performed using total RNA and hybridized with α³²P-dCTP random primer labeled human ferritin H cDNA probe. The ferritin H specific band and migration of the 18S and 28s ribosomal RNA subunits are indicated. Ethidium bromide (EtBr) staining of RNA was performed to assess equal loading. Representative images of 3 independent experiments are shown for both (A) and (B).