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Volume regulation in the two life-cycle stage of Leishmania expressing either wild-type or altered LmAQP1. (A) L. donovani promastigotes, and (B) L. donovani amastigotes expressing the vector, (●); wild-type (○); E143A/E145A (▼); E152A (△); E152D (■); and E152Q (□) LmAQP1 were subjected to a hypoosmotic shock, and the relative changes in cell volume were followed by monitoring the absorbance at 550 nm, as described in Experimental procedures.
