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. Author manuscript; available in PMC: 2009 Jun 29.
Published in final edited form as: Mol Microbiol. 2008 Oct 22;70(6):1477–1486. doi: 10.1111/j.1365-2958.2008.06494.x

Table I.

Metalloid sensitivity of L. donovani expressing altered LmAQP1

LmAQP1 EC50
[As(III)] μM [Sb(III)] μM [Sb(V)] μg/ml
vector only 18 ± 1 145 ± 45 245 ± 40
wild type 0.3 ± 0.02 0.4 ± 0.05 48 ± 4
E143A/E145A 0.3 ± 0.04 0.4 ± 0.1 50 ± 5
E152A 1.6 ± 0.2 70 ± 8 220 ± 50
E152D 0.7 ± 0.2 8 ± 3 90 ± 6
E152Q 0.3 ± 0.04 2.2 ± 0.7 65 ± 8

EC50 values for As(III) and Sb(III) were determined in L. donovani promastigotes transfected with wild type, E143A/E145A, E152A, E152D, E152Q LmAQP1 or vector control. Values for Sb(V) were determined for intracellular amastigotes.