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. Author manuscript; available in PMC: 2009 Jun 29.
Published in final edited form as: J Neuroimmunol. 2008 Apr 10;195(1-2):140–145. doi: 10.1016/j.jneuroim.2008.01.002

Figure 1. IL-12 and IL-23 production by CD46-activated mDCs.

Figure 1

(A) The production of IL-12 and IL-23 by CD46-activated mDCs was quantified by qPCR. Isolated mDCs from 10 healthy donors were matured in presence of LPS in presence or absence of anti-CD46 antibodies. After 48h, RNA were isolated and cytokine production examined by qPCR. (B) The amounts of IL-12p40 and IL-12 p70 secreted by mDC in presence of absence of CD46 were also quantified by ELISA (C) The amount of IL-17 produced by activated T cells cultured in presence of supernatants from mature mDCs with or without anti-CD46 was determined by ELISA. The addition of sIL-23R in the T cell culture blocked IL-17 induction.