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IGS of AT-10 using electrophoretic separation. AT-10 was tagged with the fluorophore 2-aminobenzoic acid, subjected to partial nitrous acid cleavage, and digested with combinations of exoenzymes as described in Materials and Methods. The resulting products were separated on a 16-cm 33% Tris-acetate gel and imaged on a UV transilluminator. The positions of standard di-, tetra-, and hexasaccharides were as shown.
