Table 1.
Derivation of PEN for disaccharide units used in this study
| I/G | 2X | 6X | 3X | NX | Hex | Disacc | Mass |
|---|---|---|---|---|---|---|---|
| 0 | 0 | 1 | 0 | 0 | 4 | I-HNAc,6S | 459.4 |
| 0 | 0 | 1 | 0 | 1 | 5 | I-HNS,6S | 497.4 |
| 0 | 1 | 1 | 0 | 1 | D | I2S-HNS,6S | 577.5 |
| 1 | 0 | 1 | 0 | 1 | −5 | G–HNS,6S | 497.4 |
| 1 | 0 | 1 | 1 | 1 | −7 | G–HNS,3S,6S | 577.5 |
The hexadecimal code derived for the disaccharide units occurring in AT-10. Column 1 is the binary position that codes for the isomeric state of the uronic acid. Columns 2–5 code for the modifications at the 2-O, 6-O, 3-O, and N positions of the disaccharide unit. Column 6 shows the hexadecimal codes represented by the binary digits in columns 2–5. Column 7 shows the disaccharide unit represented by the code in column 6. Column 8 shows the calculated theoretical masses of the disaccharide unit present internally in a sequence. For chemical or enzymatic modifications to these disaccharides, the following nomenclature is used: uronic acid with a Δ4,5 unsaturated linkage (ΔU) = ±; reducing end disaccharide unit with a mass tag =
; disaccharide unit with a five-membered anhydromannose ring = ′.