Figure 6.

Functional analysis of the AT-10 decasaccharide and comparison to the AT-III binding pentasaccharide. The in vitro anticoagulant activity of the AT-10 decasaccharide (▴) was compared with both the synthetic pentasaccharide (■) or enoxaparin (○), a LMWH generated through chemical cleavage of heparin. The activities of the three compounds was assessed by measuring either (A) anti-IIa activity, (B) anti-Xa activity, (C) anti-Xa activity using purified FXa, or (D) via HepTest. Also the activated partial thromboplastin time and the prothrombin time was measured wherein none of the compounds displayed significant activity, consistent with their high ratio of anti-Xa/anti-IIa activity (data not shown).