Table 1.
Chemical structures and m/z values of the HLGAG oligosaccharides
| Complex (M + H)+ | Saccharide (mass) | Chemical structure | Mass (calculated) |
|---|---|---|---|
| 5842.0 | 1615.2 | ΔU2SHNS,6SIHNAc,6SGHNS,3S,6S (Fig. 4A) | 1614.3 |
| 5735.1 | 1508.3 | HNS,6SGHNS,3S,6SI2SHNS,6S,OMe (Fig. 2C) | 1508.2 |
| 5655.1 | 1428.3 | HNS,6SGHNS,6SI2SHNS,6S,OMe (Fig. 3C) | 1428.1 |
| 5316.3 | 1089.5 | ΔUHNS,6SI2SHNS,6S,OMe (Fig. 3C) | 1088.9 |
| 5266.3, 5264.2 | 1036.5, 1036.1 | ΔUHNAc,6SGHNS,3S,6S (Fig. 4B and C) | 1036.9 |
| 5064.1 | 837.3 | HNS,6SGHNS,6S (Fig. 3A) | 836.7 |
| 5143.4, 5144.0 | 916.7, 917.3 | HNS,6SGHNS,3S,6S (Fig. 2A and B) | 916.8 |
| 4818.0, 4818.0, 4818.7, 4818.7 | 591.3, 591.3, 591.9, 592.0 | ΔU2SHNS,6S,OMe (Figs. 2A and B and 3A and B) | 591.5 |
| 4807.2, 4805.9 | 577.4, 577.8 | ΔU2SHNS,6S (Fig. 4B and C) | 577.5 |
Shown in column 1 is the m/z value of the protonated 1:1 complex of the saccharide and the basic peptide (RG)19R. Column 2 shows the observed mass of the saccharide obtained by subtracting the mass of protonated basic peptide from the protonated 1:1 complex. The chemical structures of the saccharides for the corresponding peaks in the mass spectra are shown in column 3. Column 4 shows the theoretical masses calculated for the chemical structures. Note that the observed mass is within ± 1 Da of the calculated mass.