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. Author manuscript; available in PMC: 2009 Jun 29.
Published in final edited form as: Biotechniques. 2007 Nov;43(5):602–passim. doi: 10.2144/000112623

Figure 3. Application of the continuous peptide methylation assay to determine the Michaelis constant (Km) value of Dim-5 for S-adenosyl-l-methionine (AdoMet).

Figure 3

Peptide methylation experiments were performed at different AdoMet concentrations ranging from 11 to 370 nM. The wells were coated with 320 pmol peptide, and the concentration of Dim-5 was 3.5 nM. Initial slopes were determined, plotted against the AdoMet concentration, and the data analyzed with respect to Km and maximal velocity (Vmax) values (insert).