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. Author manuscript; available in PMC: 2009 Jun 29.
Published in final edited form as: Cell Calcium. 2007 Sep 29;43(5):515–519. doi: 10.1016/j.ceca.2007.08.004

Figure 3. Dose-response with recombinant calcineurin.

Figure 3

A) Calcineurin assays were performs with increasing amounts of recombinant calcineurin in the presence or absence of calcium. Recombinant calcineurin resulted in increased amounts of dephosphorylated peptide in a dose-dependent manner. This activity was dependent upon calcium. Data shown are the mean +/− SEM of triplicate reactions. B) Reactions containing 0.2 ng of recombinant calcineurin were carried out along with standard controls to verify calcineurin activity. Heat inactivation of the enzyme, addition of an autoinhibitory peptide, or absence of calcium all significantly reduced activity (ANOVA). Data shown are the mean +/− SEM of triplicate samples compared to a standard curve.