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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: Mech Dev. 2009 Apr 5;126(7):552–562. doi: 10.1016/j.mod.2009.03.008

Figure 2.

Figure 2

Modified Ind proteins have altered ability to repress expression of Ind target msh in vivo. A) Ind deletion constructs. Deletion constructs of each of the conserved domains with significant sequence similarity among the Ind genes from different species were generated. IndΔEh results in removal of the N-terminal, domain. IndΔPst removes the second domain showing significant sequence identity. B–F) Antibody stains showing expression of Msh protein on stage nine embryos. Brackets indicate approximate location of Kr Gal4 expression domain B) Wild type. C) KrGal4 UAS ind, Full length Ind strongly represses Msh expression, bracket indicates Kr expression domain. D) KrGal4 UAS indΔEh no repression of Msh is observed. E) KrGal4 UAS indΔPst, has reduced ability to repress Msh. F)KrGal4 UAS indΔC, retains ability to repress Msh expression. G-K) vnd mRNA expression, stage 10 embryos brackets indicate approximate location of Kr-gal4 expression domain. G) Wild type vnd mRNA. H) KrGal4 UAS ind, Full length Ind strongly represses vnd expression. we) KrGal4 UAS indΔEh no repression of vnd is observed. E) KrGal4 UAS indΔPst, has reduced ability to repress vnd. F)KrGal4 UAS indΔC, retains ability to repress vnd expression.