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Protein markers were spiked at different concentrations in 15 yeast total cell lysate samples. Each lysate was analyzed by MudPIT (1.2) and protein identification carried out by Pep_Prob (1.3) and post-processed by DTASelect. Three different test sets were then generated. Combinations of normalization / feature selection methods were used to search for the spiked protein markers with different concentrations in each test set (1.4).
