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. Author manuscript; available in PMC: 2009 Jun 29.
Published in final edited form as: Cell Host Microbe. 2008 Feb 14;3(2):67–76. doi: 10.1016/j.chom.2007.12.008

Figure 6. LTβR-Expressing Splenic Stromal Cells Promote IFNαβ Production during MCMV Infection via a NIK-Dependent Pathway.

Figure 6

(A) Various bone marrow chimeric mice (e.g., irradiated wild-type mice receiving LTβR−/− bone marrow = LTβR→B6) were infected with MCMV for 8 hr (1 × 105 PFU i.v.). Splenic IFNβ (top panel) and ie1 (middle panel) mRNA expression was determined by qPCR and normalized to 18S rRNA levels. The ratio of IFNβ/IE1 mRNA (×100) is shown in the bottom panel. Statistical significance (*p < 0.05) was determined using the Student's t test comparing each experimental group with the B6→B6 control mice, and the fold difference is shown in parentheses. n = 3−5 mice per group ± SEM.

(B) Stromal cells from MCMV (2 × 105 PFU i.v.) infected spleens (pooled from 5 infected mice) were separated from hematopoietic cells (see Experimental Procedures). Total cell RNA was then isolated from either the stromal or hematopoietic cell fractions, and qPCR was performed to determine IFNβ, INFα, and ie1 mRNA levels. (C) aly/aly mice or wild-type littermate controls (n = 4) were infected with MCMV, and spleens were analyzed by qPCR for INFαβ and ie1 mRNA levels at 8 hr postinfection. Infection conditions and data analysis is identical to that in (A).