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. Author manuscript; available in PMC: 2009 Jun 29.
Published in final edited form as: Dev Biol. 2007 Mar 16;306(1):170–178. doi: 10.1016/j.ydbio.2007.03.014

Fig. 1.

Fig. 1

Dkk1GFP5 suppresses the activity of the β-catenin responsive reporter SuperTOPFlash in frog embryos. (A) Schematic representation of the injected constructs. Designs of constructs are described in Materials and Methods. (B) SuperTOPFlash reporter activation after injection of 250 pg CMV-GFP5 was compared to the activation occurring after injection of 250 pg CMV-Dkk1GFP5 with or without co-injection of 250 pg Xenopus CMV-Wnt3a DNA. Dkk1GFP5 suppressed both the endogenous activity of SuperTOPFlash (left two lanes) and the Wnt-3a-induced activation of SuperTOPFlash (right two lanes) in embryos. Firefly luciferase activity of the SuperTOPFlash reporter was normalized to renilla luciferase control activity. Error bars indicate the standard deviation from the mean (n=3).