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. 2000 Sep 5;97(19):10377–10382. doi: 10.1073/pnas.180317197

Figure 2.

Figure 2

Spectra of the used dyes after two-photon excitation, measured by a fiber-coupled spectrometer within the focal spot. Excitation wavelength is 830 nm. (1) Rhodamine green, (2) Texas red. The spectra are not notably different compared with one-photon excitation at the respective absorption maxima. The transmission characteristics of dichroic mirror (3) and filters [(4) and (5)] used for spectral separation of the emission light are also indicated.