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. Author manuscript; available in PMC: 2009 Sep 26.
Published in final edited form as: Mol Cell. 2008 Sep 26;31(6):813–823. doi: 10.1016/j.molcel.2008.07.022

Figure 2. Internal TR Sequences Are Dispensable for Homing.

Figure 2

(A) TR deletion constructs and homing activities. All donor plasmids are derivatives of pMX1b, containing AvrII (Av) and ApaI (Ap) sites in atd and brt, respectively, as well as a sequence tag (TG2). AvrII and ApaI sites were introduced via silent mutations and do not affect DGR homing (Figure S6). FL (full-length) is a positive control with TG2 inserted at position 84. ΔTR1-84, ΔTR11-84, ΔTR23-84, ΔTR33-84, ΔTR33-96 and ΔTR33-113 contain TR deletions with TG2 at deletion junctions. Results from PCR homing assays are summarized: +, homing efficiency similar to the FL positive control; ++, homing activity greater than FL; −, no homing activity detected.

(B) Primers used for homing assays. P5 and P6 are sense- and antisense-strand primers annealing to TG2, respectively; primers P1 and P2 are described in Figure 1.

(C) PCR homing assays with TR deletion constructs. Homing assays were performed following BPP-1d single-cycle lytic infection of RB50 cells transformed with the indicated plasmids. *, Brt-independent PCR artifacts.