Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2010 Mar 27.

Published in final edited form as: Neurosci Lett. 2009 Feb 4;453(1):68–72. doi: 10.1016/j.neulet.2009.01.077

We demonstrated mRNA and protein expression of AQP4 and 9 at control, 4 hours post-TBI, and 4 hours post-TBI with injection of 2ME2 levels. Real time PCR analysis demonstrated a significant reversal (p<0.05, indicated by #) of AQP4 (A) and AQP9 (B) gene up-regulation due to TBI (p<0.05, indicated by *) when 2ME2 is administered to the rats. Transcription of AQP4 and AQP9 returned to near control levels. Protein expression of AQP4 (C) and AQP9 (D) are significantly (p<0.05, indicated by *) increased, but intravenous injection of 2ME2 30 minutes post-TBI significantly (p<0.05, indicated by #) reversed those changes and kept AQP4 and AQP9 expression at or below control levels. Protein equal loading was confirmed by intracellular protein β-actin. Representative immunoblots are presented. Values are mean +/− SE.

We demonstrated mRNA and protein expression of AQP4 and 9 at control, 4 hours post-TBI, and 4 hours post-TBI with injection of 2ME2 levels. Real time PCR analysis demonstrated a significant reversal (p<0.05, indicated by #) of AQP4 (A) and AQP9 (B) gene up-regulation due to TBI (p<0.05, indicated by *) when 2ME2 is administered to the rats. Transcription of AQP4 and AQP9 returned to near control levels. Protein expression of AQP4 (C) and AQP9 (D) are significantly (p<0.05, indicated by *) increased, but intravenous injection of 2ME2 30 minutes post-TBI significantly (p<0.05, indicated by #) reversed those changes and kept AQP4 and AQP9 expression at or below control levels. Protein equal loading was confirmed by intracellular protein β-actin. Representative immunoblots are presented. Values are mean +/− SE.