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. Author manuscript; available in PMC: 2009 Dec 16.
Published in final edited form as: Biochemistry. 2008 Dec 16;47(50):13354–13361. doi: 10.1021/bi8017762

Fig. 4.

Fig. 4

Running start experiment with pol η on an abasic site. (A) Schematic diagram of the 31/68-mer substrate used in the running start assays with the ends of the template strand containing biotinstreptavidin blocks. The X indicates the location of the abasic site (B) Autoradiograph of the synthesis products after five or fifteen minutes following the addition of pol η. The arrow indicates incorporation opposite the abasic site. Lanes labeled WT contain the wild-type PCNA protein, and lanes labeled MT contain the G178S mutant PCNA protein.

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