Fig. 5.

Steady state kinetics of pol η on an abasic site. The rate of nucleotide incorporation was graphed as a function of dGTP concentration for (A) pol η alone, (B) pol η with wild type PCNA protein, (C) and pol η with the G178S PCNA mutant protein. Autoradiographs of the gels showing the incorporation of a single dGTP across from an abasic site at the indicated concentrations of nucleotide are shown above each graph. The solid lines represent the best fits of the data to the Michaelis–Menten equation, and the V_max and K_m steady state parameters are given in Table 2.