Table 2.
Steady state kinetic parameters of pol η–catalyzed nucleotide incorporation.
| Proteins | DNA |
Vmax (nM/min) |
Km (μM) |
Vmax/Km |
Relative efficiency |
|---|---|---|---|---|---|
| Pol η alone | Abasic site | 1.0 ± 0.1 | 34 ± 8 | 0.029 | 1.0 |
| Pol η + wild-type PCNA | Abasic site | 0.70 ± 0.06 | 9.9 ± 3.0 | 0.071 | 2.4 |
| Pol η + mutant PCNA | Abasic site | 0.12 ± 0.01 | 12 ± 1 | 0.010 | 0.34 |
| Pol η alone | Non-damaged | 3.2 ± 0.2 | 0.67 ± 0.12 | 4.8 | 1.0 |
| Pol η + wild-type PCNA | Non-damaged | 4.8 ± 0.3 | 0.32 ± 0.07 | 15 | 3.1 |
| Pol η + mutant PCNA | Non-damaged | 3.4 ± 0.3 | 2.1 ± 0.4 | 1.6 | 0.33 |