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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Mol Cancer Ther. 2009 Mar 10;8(3):499–508. doi: 10.1158/1535-7163.MCT-08-0544

Fig. 1.

Fig. 1

Comparison of the morphology of P69, M12, F6 and M12-Vim prostate sublines grown on tissue culture dishes (2D) versus embedded in lrECM gels (3D). (A) Light microscopy images of these various prostate cancer sublines were taken from cultures grown in 2D on traditional plastic dishes for 4 days versus 8 days embedded in lrECM (3D) as described in Materials and Methods. Magnification is at 10X. (B) Whole cell extracts (40 µg) from 2D and 3D cultures of these sublines were subjected to Western blot analysis with vimentin antibody as described in Materials and Methods. β-actin was used as loading control.