Fig. 5.

Inclusion of blocking antibodies to β1-integrin (AIIB2) or α6-integrin (GoH3) leads to the formation of reverted acini with the metastatic M12 subline. (A) Light microscopy images of the M12 and F6 subline grown embedded in lrECM for 10 days in the absence or presence of inhibitory antibodies or IgG as a negative control are noted. Magnification is at 20X. (B) Confocal immunofluorescence microscopy of the M12 or F6 subline grown embedded in lrECM for 10 days in the absence or presence of AIIB2, GoH3 or IgG as indicated. Cells were stained with E-cadherin (green) and β-catenin (red) and nuclei were stained with DAPI (blue). Magnification is at 63X and a size marker of 5 µm is shown. (C) Whole cell extracts (40 µg) from 10 day cultures as described in panel A were subjected to Western blot analysis with vimentin, β1-integrin, or β-actin antibody.