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. 2009 Mar 19;150(7):3145–3152. doi: 10.1210/en.2008-1740

Figure 4.

Figure 4

Effect of chronic GH treatment on the expression of IGF-I and IGFBP-3 mRNA in extrahepatic tissues. WT and null ALS mice (null) were treated twice daily with GH (3 μg/g body weight per injection) or an equivalent volume of saline from 35 to 63 d of age. Total RNA was extracted from skeletal muscle, adipose tissue, or kidney at the end of treatment and analyzed by real-time quantitative RT-PCR for abundance of IGF-I mRNA (A) or IGFBP-3 mRNA (B). Results are expressed relative to the mean expression level of saline-treated WT mice. The significant effects of G (WT or null), H (saline or GH), and their interaction (G × H) are reported. Each bar represents the mean ± se of seven to nine female mice.