Development of kisspeptin-10 immunoreactivity in the AVPV, rPVpo, and cPVpo of P10, P15, P20, P25, P30, and P60 female mice. A–C, Representative sections of kisspeptin immunoreactivity in the AVPV, rPVpo, and cPVpo. D, Histogram shows the mean (+sem) number of kisspeptin cell bodies per section at the three levels of the RP3V across postnatal development. Bars with different letters are significantly different from one another (P < 0.05; ANOVA with Student-Newman-Keuls post hoc test). Scale bar in A–C is 200 μm.