Ovarian steroids control the developmental increase in kisspeptin immunoreactivity in the RP3V. A and B, Kisspeptin-10 immunoreactivity in the rPVpo of sham (A) and P15 OVX (B) mice killed at P30. C and D, Quantitative analysis of kisspeptin immunoreactive cell bodies in the AVPV, rPVpo, and cPVpo of the RP3V given as the mean (+sem) number of immunoreactive cell bodies detected per section in P15 OVX and sham-treated mice killed at P30 (n = 4 per group) (C) and P60 (n = 6 per group) (D). ***, P < 0.001 compared with respective sham. Scale bars, 100 μm. 3V, Third ventricle.