Figure 6.

OSAP knockdown decreases mitochondrial DNA abundance per cell. Relative amounts of mitochondrial DNA (mtDNA) and nuclear DNA (nucDNA) in Y-1 cells, 48 h after transfection with control or OSAP siRNA, were determined by qRT-PCR as described in Materials and Methods. The mtDNA to nucDNA ratio, an index of mtDNA concentration per cell, was significantly decreased in OSAP siRNA cells compared with control siRNA cells. For comparison, the relative mtDNA to nucDNA ratio of the control is arbitrarily presented as 1. Data shown are the mean ± se of four determinations from one representative experiment. *, P < 0.01 by Student’s t test (vs. control siRNA cells).