Figure 3.

Location of PRE and coRE on 12 PR-up-regulated promoters and other promoter constructs. A, SRMS was used to locate 2000-nt proximal promoters of 12 PR-regulated genes, which were searched for PRE using the TRANSFAC database 16-nt consensus PRE V$GRE_C matrix (open red boxes) or the 19-nt experimental GRE V$Gr_Q6 matrix (open yellow boxes). A highly conserved pattern of seven motifs, each approximately 16 nt in length (each colored box), spanning about 234 bp total, was found on 11 of the 12 promoters. The 234-nt sequence is termed a “coRE”. Location of each coRE on the sense (+) and antisense (−) strand is indicated. The numbering on the abscissa is the reverse of standard promoter nomenclature with 2000 at the transcription start site, and 0 at −2000 bp. B, Luciferase reporters driven by promoters containing PRE and the coRE of the OGG1 promoter, cloned in the forward or reverse orientation as it aligns in OGG1, were made as described in Materials and Methods. Open red boxes denote ½, one, or two palindromic PRE derived from the TAT promoter with each half-site separated by a dashed line. Mutated sites are designated by X. Also shown are constructs containing the three half-sites of the MMTV-LTR. Open black box is the TATA box of the minimal tk promoter.