Figure 5.

Effects of the coRE on transcription by other nuclear receptors. A, The PRE2-tk-LUC reporter without or with the coRE in the forward or reverse orientation was transiently transfected into HeLa cells together with a Renilla-LUC control, and cells were treated 24 h with 10 nm dexamethasone. B, The same reporters were transiently transfected into HeLa cells together with an expression plasmid encoding AR and the Renilla-LUC control. Cells were treated 24 h with 10 nm of the androgen R1881. C, The coRE in either orientation was cloned upstream of an ERE2-tk-LUC reporter, HeLa cells were transiently transfected with the reporter, an ERα expression vector, and Renilla-LUC and treated 24 h with 10 nm 17β-estradiol. Fold changes are shown for hormone treated vs. untreated transcription, corrected for the Renilla control. Error bars represent the sem of three independent studies.