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A PRB dimerization mutant monomer up-regulates transcription of endogenous genes. T47D breast cancer cell lines that stably express wild-type PRB (YB) or PRB dimerization mutants (DBX136; DBX141), were treated 6 h without (−) or with (+) 10 nm progesterone and harvested and RNA was isolated. RT-PCR was performed with primers specific for tissue factor (F3), PNMT, or SGK. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is shown as a loading control. Prog, Progesterone.
