Figure 7.

Compared with wild-type PRB, DBX monomers strongly stimulate PRE-dependent transcription, which is modified by the coRE. A, HeLa cells were transiently transfected with the single or tandem PRE half-site tk-LUC, or single or tandem palindromic PRE tk-LUC, plus wild-type PRB or DBX dimerization mutants, and a Renilla-LUC vector. Cells were treated 24 h without or with 10 nm R5020. Luciferase activity was corrected for the Renilla control, and fold regulation by liganded vs. unliganded receptors was quantified. B, HeLa cells were transiently transfected with the single or tandem palindromic PRE tk-LUC without or with the coRE in the fwd or rev orientation. They were cotransfected with wild-type PRB or DBX expression vectors, and a Renilla-LUC vector, treated 24 h without or with 10 nm R5020, and quantified as above. C, HeLa cells were transiently transfected with the single or tandem PRE half-site tk-LUC without or with the coRE in the fwd or rev orientation. They were cotransfected with PRB or DBX expression vectors and a Renilla-LUC vector and treated 24 h without or with 10 nm R5020. Transcription was quantified as above. Error bars represent the sem of three independent studies.