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. 2009 Apr 2;23(7):966–974. doi: 10.1210/me.2008-0308

Figure 3.

Figure 3

Regulation of LXR target gene expression in DN2-overexpressing macrophages. A, Peritoneal macrophages were isolated from wild-type (WT) and DN2-Tg mice and treated for 24 h with vehicle [dimethylsulfoxide (DMSO)] or 1 μm T0901317 (T1317). mRNA levels of ABCA1, ABCG1, and apoE were measured using QPCR. B, Peritoneal macrophages were isolated from wild-type mice, transfected with DN2 and ASC-2, and treated with vehicle or T0901317. Total RNA was extracted and subjected to QPCR to assay for levels of ABCA1, ABCG1, and apoE mRNA. C, Peritoneal macrophages were isolated from mice, transfected with DN2 or pcDNA3, and incubated with 50 μg/ml acLDL and 1 μm T0901317 for 24 h. QPCR was performed to measure the levels of ABCA1, ABCG1, and apoE mRNA in acLDL-laden foam cells. *, P < 0.05; **, P < 0.005; ***, P < 0.001.