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. 2009 Apr 2;23(7):966–974. doi: 10.1210/me.2008-0308

Figure 5.

Figure 5

Effects of DN2 on the recruitment of ASC-2, LXR, TRAP220, and NCoR to the LXREs in the ABCA1, ABCG1, and apoE promoters in peritoneal macrophages. Peritoneal macrophages were isolated from thioglycolate-elicited mice and transfected with DN2. After treatment with vehicle or T0901317, ChIP analyses were performed using ASC-2 (A300-411A; Bethyl), LXR (sc-13068; Santa Cruz), TRAP220 (sc-5334; Santa Cruz), or NCoR (sc-1609X; Santa Cruz) antibodies. The background immunoprecipitation with preimmune IgG was also performed. Immunoprecipitated LXRE-containing DNA levels were quantitated using QPCR and normalized to input DNA. As a control, the non-LXRE region of each promoter was amplified using each immunoprecipitate. **, P < 0.005; ***, P < 0.001. DMSO, Dimethylsulfoxide.