Figure 1.

Localization of GOS 28 and Syntaxin 5 in the Golgi complex of CHO or HeLa cells. Shown are representative cryosections of Golgi complexes cut in a cis–-trans orientation. The gold particles revealing GOS 28 are distributed across the stack with a preferential localization on the periphery (buds and vesicles) of the cisternae (A and B). The gold particles labeling Syntaxin 5 (Syn5) show a more central distribution on the cisternae and appear slightly more concentrated on the cis side of the stack (C and D). The five cisternae comprised in a typical stack are numbered in panels B and D (1 = cis-most; 5 = trans-most). The quantitative evaluation of the respective labeling over the Golgi cisternae and lateral vesicles is shown in Figs. 2 and 3. A and B are CHO cells; C and D are HeLa cells. RER, rough ER. (Bars represent 0.2 μm.)