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. 2000 Aug 29;97(19):10400–10405. doi: 10.1073/pnas.190292497

Figure 4.

Figure 4

Double immunolabeling of GOS 28 and KDEL receptor (KDELr), or GOS 28 and VSV-G in the Golgi complex of CHO cells. The small gold particles revealing GOS 28 show no colocalization with large gold particles revealing KDELr on the cisternal buds and vesicles (A and B). Colocalization of GOS 28 and KDELr is detectable only on the stacked cisternae (A and Inset in A). The large gold particles revealing GOS 28 colocalize with the small gold particles labeling VSV-G protein on cisternae, coated buds (upper Inset in C), or vesicles (lower Inset in C). The quantitative evaluation of the labeling is shown in Table 2. [The bar shown in C (0.2 μm) applies for the upper Inset in C and for A and B. The bar shown in the Inset in A (0.1 μm) applies for the lower Inset in C.]