Table 1.

Distribution of GOS 28 over the Golgi complex in CHO/F3 cells

Compartment	Surface density of gold labeling, particles/μm2	% of compartment labeled	Distribution of gold, % of total
1.  Cisternae of Golgi stack	53  ± 6		49
2.  Cis half	60  ± 9		48  ± 5
3.  Trans half	49  ± 8		52  ± 5
4.  Vesicles/buds on the cis side of the stack	70  ± 16	26  ± 5	14
5.  Vesicles/buds lateral to the stack	90  ± 7	33  ± 3	37
6.  Vesicles lateral to the stack	92  ± 7	33  ± 2
7.  Buds lateral to the stack	95  ± 21	34  ± 7
8.  Vesicles cis half	84  ± 15	31  ± 6
9.  Vesicles trans half	100  ± 14	34  ± 4
10.  Buds cis half	111  ± 33	33  ± 11
11.  Buds trans half	94  ± 24	36  ± 9

CHO/F3 cells expressing myc-GOS 28. Thin sections were immunolabeled for GOS 28 with affinity-purified rabbit anti-GOS 28 (diluted 1:3), followed by goat anti-rabbit IgG gold (15-nm gold). Values are mean ± SEM from 20 different Golgi complexes.