Table 2.

Simultaneous localization of KDEL receptor and GOS 28 over the Golgi complex in CHO/F3 cells

70- to 90-nm diameter vesicle labeling	% vesicles located
	On cis side of stack	On lateral side of stack
KDEL receptor only	28  ± 5	25  ± 3
myc-GOS 28 only	16  ± 3	22  ± 3
Both proteins	0	2  ± 1

CHO/F3 cells expressing myc-GOS 28. Thin sections were double-labeled for KDEL receptor and GOS 28 with rabbit polyclonal anti-KDEL receptor and mouse monoclonal anti-myc 9E10 antibodies, followed by goat anti-rabbit IgG gold (15-nm gold) and goat anti-mouse IgG gold (10-nm gold). The quantitative evaluation was performed on 20 separate Golgi areas. Data are mean ± SEM.