Table 3.

Simultaneous localization of VSV G protein and GOS 28 in CHO/F3 cells

70- to 90-nm diameter vesicles and buds on the lateral side of stack labeling	% of vesicles/buds labeled	% of VSV G-positive vesicles/buds also staining for GOS 28
VSV G only	18  ± 2	—
GOS 28 only	21  ± 2	—
Both proteins	11  ± 1	44  ± 4

CHO/F3 cells were infected with VSV for 1 h then incubated with VSV-free medium for 2 h before fixation. Thin sections were double-labeled with mouse monoclonal anti-VSV G protein antibodies (P5D4) and affinity-purified rabbit anti-GOS 28 (diluted 1:5), followed by goat anti-mouse IgG gold (10-nm gold) and goat anti-rabbit IgG gold (15-nm gold). The quantitative evaluation was performed on 40 Golgi areas at a magnification of ×72,000. Data are mean ± SEM.