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Total protein extracts were obtained from adult flies and probed with an anti-H3K9me2 specific antibody; anti-H4 was used as a loading control. A stock carrying a null mutation of Su(var)3-9 histone methyltransferase was used as a control. Vig2PL470 demonstrates a diminished level of H3K9me2, whereas vigEP812 does not. Error bars represent standard error of the mean.
