Table 3.

Summary of baculovirus purification from hemolymph for 25 number of silkworm larvae harvested at different post-injection

Harvesting time (p.i.)	Volume (ml)	Titer (pfu/ml)	Virus (pfu)	Protein (mg/ml)	Virus/protein (pfu/mg)	Recovery (%)	Purification (fold)
4d
Hemolymph	16	2.03E+08	3.25E+09	34.5	5.88E+06	100	1
Concentrated virus after Sephacryl	0.9	1.35E+08	1.22E+08	0.087	1.55E+09	4	264
4.5d
Hemolymph	16	4.22E+08	6.75E+09	34.9	1.21E+07	100	1
Concentrated virus after Sephacryl	0.9	9.96E+07	8.96E+07	0.237	4.20E+08	1	35
5d
Hemolymph	12	8.29E+08	9.95E+09	43.2	1.91E+07	100	1
Concentrated virus after Sephacryl	0.6	4.90E+08	2.94E+08	0.659	7.43E+08	3	39