Table 1.
M lineage phenotypes of C. elegans Pax-Six-Eya-Dac mutants
Gene family | Genotype | Number of Embryonic CCs | Number of M-derived CCs |
---|---|---|---|
wild-type | 4 (n>200) | 2 (n>200) | |
ceh-34(RNAi-P) | 4 (n>200) | 0 (98.3%); 2 (1.7%) (n=362) |
|
Six1/2 | ceh-34(RNAi-E) | 11.3% with 1st pair missing 16.7% with 2nd pair missing 14.1% with both pairs missing (n=538) |
0 (98.6%) 1-2 (1.4%) (n=425) |
ceh-33(RNAi-P) | 4 (n>200) | 2 (n>200) | |
Six3/6 | ceh-32(RNAi-P) | 4 (n>200) | 2 (n>200) |
Six4/5 | ceh-35/unc-39 (RNAi-P) | 4 (n>200) | 2 (n>200) |
Eyes absent | eya-1(ok654) | 9.5% with 1st or 2nd pair missing 87.7% with both pairs missing (n=203) |
0 (84.7%);1 (1.0%); 2 (14.3%) (n=203) |
Pax1/9 | pax-1(RNAi-P) | 4 (n>200) | 2 (n>200) |
Pax2/5/8 | pax-2(RNAi-P) | 4 (n>200) | 2 (n>200) |
egl-38(RNAi-P) | 4 (n>200) | 2 (n>200) | |
egl-38(n578) | 4 (n>200) | 2 (n>200) | |
Pax3 | pax-3(RNAi-P) | 4 (n>200) | 2 (n>200) |
Pax6 | vab-3(RNAi-P) | 4 (n>200) | 2 (n>200) |
Dachshund | dac-1(gk211) | 4 (n>200) | 2 (n>200) |
dac-1(gk213) | 4 (n>200) | 2 (n>200) |
RNAi-P: RNAi was administered to synchronized L1 larvae via feeding throughout post-embryonic development.
RNAi-E: RNAi via injection into the gonads of gravid adults whose progeny were scored for phenotypes.