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. Author manuscript; available in PMC: 2010 Jul 31.
Published in final edited form as: J Immunol Methods. 2009 May 5;346(1-2):18–25. doi: 10.1016/j.jim.2009.04.013

Figure 4. Effect of serum dilution on ELISA sensitivity.

Figure 4

Samples consisted of a BP serum with high levels of both IgG and IgE, a BP serum with intermediate (med) levels of both antibody isotypes and a (neg) control. Sera were undiluted or serially diluted 1 to 10, 20, 40, 80 and 100. Index values are shown for the IgG (A) ELISA used as directed (closed symbols) and OD450 is shown for dilutions developed with anti-IgE secondary antibody (open symbols). For comparison, index values over a range of dilutions are shown for the IgE ELISA protocol described in this report (B). Results are representative of two independent experiments and each data point represents the mean of duplicate samples.