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. 2000 Sep 5;97(19):10406–10411. doi: 10.1073/pnas.180321497

Figure 3.

Figure 3

Effect of c-Jun dominant negative mutant on c-Jun/Sp1 interaction in pRSVjun-treated cells. Cells were transfected with 2 μg of pRSVjun and a different amount of c-Jun dominant negative vector TAM-67 by the lipofection method. After the change of Opti-MEM medium to 3 ml of fresh culture medium in a 6-cm plastic dish, cells were incubated for an additional 36 h. Expression of c-Jun and TAM-67 proteins (A) and the coimmunoprecipitated c-Jun/Sp1 and TAM-67/Sp1 complex by using anti-Sp1 antibodies (B) was analyzed by Western blot with anti-c-Jun and anti-Sp1 antibodies. pcDNA3.1 was used as a vector to adjust for the same amount of plasmids in each experiment.