Figure 5.

Effect of EGF on the interaction between Sp1 oligonucleotides and c-Jun/Sp1. Nuclear extracts from EGF-treated cells were prepared and subjected to the assay for binding of the c-Jun/Sp1 complex to Sp1 consensus sites. The ³²P-radiolabeled Sp1 oligonucleotide (●) and Sp1 mutant SPM (□) were used as a probe for binding, respectively. For background control, protein A-agarose was used to substitute anti-c-Jun antibody–agarose conjugate in the assay (■). Values are means ± SEM of three determinations.