Skip to main content
. 2000 Sep 5;97(19):10406–10411. doi: 10.1073/pnas.180321497

Figure 6.

Figure 6

Transactivation of the 12(S)-lipoxygenase promoter consisting of ×10 GAL4-binding sites by GAL4-c-Jun in EGF-treated cells. Constructs of luciferase reporters and expression vectors used in these experiments are indicated (A). The transactivation effect of GAL4-c-Jun on a chimeric promoter bearing GAL4-binding sites was analyzed (B). Cells were transfected with 0.5 μg of pXLO-8G, 0.2 μg of β-galactosidase, 10 ng of pFA2-c-Jun, or 50 ng of pFC2dbd by the lipofection method, as indicated. After the change of Opti-MEM medium to 2 ml of fresh culture medium in a 3.5-cm plastic dish, cells were incubated for an additional 24 h and then treated with 50 ng/ml EGF. After 30 min of EGF treatment, the medium was removed, and the cells were further cultured in fresh culture medium for 6–18 h. Luciferase and β-galactosidase activities were then determined, respectively. Values are means ± SEM of three determinations.