Figure 5. The clock in the fat body affects glycogen storage.

Glycogen levels were measured in 1-week-old male flies of different genotypes. At least three independent groups of 10 adult male flies each were analyzed for each genotype. Glycogen levels were normalized to the protein concentration. (A) – (C) Glycogen levels are lower in ,males carrying both UAS-dnCLK and to-Gal4 (A), male flies carrying UAS-dnCYC and to-Gal4 (B) and Female flies carrying UAS-dnCLK and yolk-Gal4 (C). (D) Glycogen levels are normal in Clk^jrk flies and equivalent to levels in control ry⁵⁰⁶ flies. Asterisks indicate significant differences (p<0.05). Statistical significance was determined by two-tailed Student’s t-test with unequal variance.