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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Microbiology (Reading). 2009 Jan;155(Pt 1):46–52. doi: 10.1099/mic.0.021048-0

Fig. 2.

Fig. 2

The transcriptional regulator Srv is required for maximal biofilm formation by MGAS5005. Each reported value for the CV assay is an average of at least six replicates and is adjusted by the dilution factor required to obtain a spectrophotometric reading (A600e). (a) A comparison of the wild-type (MGAS5005), the isogenic mutant lacking srv (Δsrv), and the complemented strain [MGAS5005Δsrv(pIAβ8-srv)]. The Δsrv mutant is significantly reduced in its ability to form biofilms. Complementation of srv in trans restores biofilm formation. The mean A600 (extrapolation unnecessary) for wells containing THY alone is provided as a negative control. (b) Kinetics of GAS biofilm formation. The A600e values of solubilized CV from surface-attached cells grown in six-well polystyrene plates were assayed over time.